Journal
CELL REPORTS
Volume 19, Issue 9, Pages 1953-1966Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2017.05.022
Keywords
-
Categories
Funding
- German Research Foundation (DFG) [MA 5831/1-1]
- European Research Council (ERC) under the European Union's Horizon research and innovation program [680042]
- German Federal Ministry of Research and Education [031A315, 031A424]
- European Research Council (ERC) [680042] Funding Source: European Research Council (ERC)
Ask authors/readers for more resources
Cell cycle kinetics are crucial to cell fate decisions. Although live imaging has provided extensive insights into this relationship at the single-cell level, the limited number of fluorescent markers that can be used in a single experiment has hindered efforts to link the dynamics of individual proteins responsible for decision making directly to cell cycle progression. Here, we present fluorescently tagged endogenous proliferating cell nuclear antigen (PCNA) as an all-in-one cell cycle reporter that allows simultaneous analysis of cell cycle progression, including the transition into quiescence, and the dynamics of individual fate determinants. We also provide an image analysis pipeline for automated segmentation, tracking, and classification of all cell cycle phases. Combining the all-in-one reporter with labeled endogenous cyclin D1 and p21 as prime examples of cell-cycle-regulated fate determinants, we show how cell cycle and quantitative protein dynamics can be simultaneously extracted to gain insights into G1 phase regulation and responses to perturbations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available