The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages

We recently demonstrated that the large Pseudomonas chlororaphis bacteriophage 201 phi 2-1 assembles a nucleus-like structure that encloses phage DNA and segregates proteins according to function, with DNA processing proteins inside and metabolic enzymes and ribosomes outside the nucleus. Here, we investigate the replication pathway of the Pseudomonas aeruginosa bacteriophages phi KZ and phi PA3. Bacteriophages phi KZ and phi PA3 encode a proteinaceous shell that assembles a nucleus-like structure that compartmentalizes proteins and DNA during viral infection. We show that the tubulin-like protein PhuZ encoded by each phage assembles a bipolar spindle that displays dynamic instability and positions the nucleus at midcell. Our results suggest that the phage spindle and nucleus play the same functional role in all three phages, 201 phi 2-1, phi KZ, and phi PA3, demonstrating that these key structures are conserved among large Pseudomonas phages.