Journal
CELL REPORTS
Volume 18, Issue 6, Pages 1473-1483Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2017.01.027
Keywords
-
Categories
Funding
- Medical Research Council [MC_UU_12010, G0902418, MC_UU_12025]
- Wolfson Foundation [18272]
- MRC/BBSRC/EPSRC [MR/K01577X/1]
- Wellcome Trust [104924/14/Z/14]
- Deutsche Forschungsgemeinschaft [1905]
- Nuffield Department of Medicine Leadership Fellowship from Oxford University
- Wellcome Trust Centre for Human Genetics is supported by Wellcome Trust [090532/Z/09/Z]
- Cellular Imaging Core from the Wellcome Trust Centre for Human Genetics
- Wolfson Imaging Centre at the Weatherall Institute of Molecular Medicine
- MRC [MR/K01577X/1, G0902418, MC_UU_00008/9, MC_UU_12010/9, G0901732, G0400453] Funding Source: UKRI
- Medical Research Council [MR/K01577X/1, G0400453, G0902418, MC_UU_00008/9, MC_UU_12010/9, G0901732] Funding Source: researchfish
Ask authors/readers for more resources
HIV-1 disseminates to diverse tissues and establishes long-lived viral reservoirs. These reservoirs include the CNS, in which macrophage-lineage cells, and as suggested by many studies, astrocytes, may be infected. Here, we have investigated astrocyte infection by HIV-1. We confirm that astrocytes trap and internalize HIV-1 particles for subsequent release but find no evidence that these particles infect the cell. Astrocyte infection was not observed by cell-free or cell-to-cell routes using diverse approaches, including luciferase and GFP reporter viruses, fixed and live-cell fusion assays, multispectral flow cytometry, and super-resolution imaging. By contrast, we observed intimate interactions between HIV-1-infected macrophages and astrocytes leading to signals that might be mistaken for astrocyte infection using less stringent approaches. These results have implications for HIV-1 infection of the CNS, viral reservoir formation, and antiretroviral therapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available