Journal
CELL REPORTS
Volume 18, Issue 4, Pages 1048-1061Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2016.12.087
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Funding
- Charles H. Hood Foundation Postdoctoral Research Fellowship
- American Heart Association Postdoctoral Fellowship [13POST14540015]
- Department of Defense [W81XWH-14-PRMRPDA]
- NIH [DK102173, DK102170, DK085171]
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Epigenomic mechanisms direct distinct gene expression programs for different cell types. Various in vivo tissues have been subjected to epigenomic analysis; however, these studies have been limited by cellular heterogeneity, resulting in composite gene expression and epigenomic profiles. Here, we introduce ''NuTRAP,'' a transgenic mouse that allows simultaneous isolation of cell-type-specific translating mRNA and chromatin from complex tissues. Using NuTRAP, we successfully characterize gene expression and epigenomic states of various adipocyte populations in vivo, revealing significant differences compared to either whole adipose tissue or in vitro adipocyte cell lines. We find that chromatin immuno-precipitation sequencing (ChIP-seq) usingNuTRAPis highly efficient, scalable, and robust with even limited cell input. We further demonstrate the general utility of NuTRAP by analyzing hepatocyte-specific epigenomic states. The NuTRAP mouse is a resource that provides a powerful system for cell-type-specific gene expression and epigenomic profiling.
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