Journal
CELL REPORTS
Volume 20, Issue 12, Pages 2935-2943Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2017.08.084
Keywords
-
Categories
Funding
- DOE Office of Biological and Environmental Research
- NIH, National Institute of General Medical Sciences (NIGMS) [P41GM103393]
- NIH [R56 AI117675]
- Skaggs Institute for Chemical Biology
- U.S. Department of Energy (DOE) Office of Science, Office of Basic Energy Sciences [DE-AC02-76SF00515]
Ask authors/readers for more resources
Antibodies that target both group 1 and group 2 influenza A viruses are valuable for therapeutic and vaccine development, but only a few have been reported to date. Here, we describe a new V(H)1-69 antibody 27F3 that broadly recognizes heterosubtypic hemagglutinins (HAs) from both group 1 and group 2 influenza A viruses. Structural characterization of 27F3 Fab with A/California/04/2009 (H1N1) hemagglutinin illustrates that 27F3 shares the key binding features observed in other V(H)1-69 antibodies to the HA stem. Compared to other V(H)1-69 antibodies, the 27F3 V-H domain interacts with the HA stem in a distinct orientation, which alters its epitope and may have influenced its breadth. The diverse rotations of V(H)1-69 antibodies on the HA stem epitope highlight the different ways that this antibody family can evolve to broadly neutralize influenza A viruses. These results have important implications for understanding how to elicit broad antibody responses against influenza virus.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available