Journal
DEVELOPMENTAL CELL
Volume 41, Issue 3, Pages 243-+Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2017.04.002
Keywords
-
Categories
Funding
- Francis Crick Institute
- Cancer Research UK [FC001051]
- UK Medical Research Council [FC001051]
- Wellcome Trust [FC001051, WT098326MA]
- BBSRC [BB/J015539/1]
- Max Delbruck Center for Molecular Medicine in the Helmholtz Association
- Wellcome Trust [098325/Z/12/Z] Funding Source: Wellcome Trust
- BBSRC [BB/J015539/1] Funding Source: UKRI
- MRC [MR/K017047/1, MR/K011200/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J015539/1] Funding Source: researchfish
- Medical Research Council [MR/K017047/1, MR/K011200/1] Funding Source: researchfish
- The Francis Crick Institute [10002] Funding Source: researchfish
- Wellcome Trust [098325/Z/12/Z] Funding Source: researchfish
Ask authors/readers for more resources
Transcriptional networks, regulated by extracellular signals, control cell fate decisions and determine the size and composition of developing tissues. One example is the network controlling bipotent neuromesodermal progenitors (NMPs) that fuel embryo elongation by generating spinal cord and trunk mesoderm tissue. Here, we use single-cell transcriptomics to identify the molecular signature of NMPs and reverse engineer the mechanism that regulates their differentiation. Together with genetic perturbations, this reveals a transcriptional network that integrates opposing retinoic acid (RA) and Wnt signals to determine the rate at which cells enter and exit the NMP state. RA, produced by newly generated mesodermal cells, provides feedback that initiates NMP generation and induces neural differentiation, thereby coordinating the production of neural and mesodermal tissue. Together, the data define a regulatory network architecture that balances the generation of different cell types from bipotential progenitors in order to facilitate orderly axis elongation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available