Journal
CHEMOSPHERE
Volume 177, Issue -, Pages 311-316Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2017.03.018
Keywords
NaClO3; Comet assay; DNA damage; DNA-protein cross-links; Genotoxicity
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Funding
- University Grants Commission, New Delhi (SAP-DRS-III)
- Department of Science and Technology (DST-FIST) [F1.-17.1/2010/MANF-MUS-WES-1902]
- UGC-MANF
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Sodium chlorate (NaClO3) is widely used in paper and pulp industries and as a non-selective herbicide. It is also a major by-product generated upon disinfection of drinking water by chlorine dioxide. In this study, we have investigated the genotoxicity of NaClO3 on the small intestine of rats. Adult male rats were divided into 5 groups: one control and four NaClO3 treated groups. The NaClO3 treated groups were given a single acute oral dose of NaClO3 (100, 250, 500 and 750 mg/kg body weight) and sacrificed 24 h later. Administration of NaClO3 caused significant DNA damage in a dose dependent manner in the rat intestine. This was evident from the comet assay which showed DNA strand breaks and was further confirmed by agarose gel electrophoresis and release of free nucleotides. Increased DNA protein cross linking in NaClO3 administered groups showed formation of a critical lesion which hampers activities of proteins/enzymes involved in DNA repair, transcription and replication. Thus, oral administration of NaClO3 induces DNA damage in the rat intestine, probably through chlorate induced production of reactive oxygen species. (C) 2017 Elsevier Ltd. All rights reserved.
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