Journal
ANALYTICA CHIMICA ACTA
Volume 969, Issue -, Pages 57-62Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2017.02.040
Keywords
Carbon nanotube/Gold nanoparticles nanocomposite probe; Cotton thread; Point-of-care diagnosis; Immunochromatographic assay
Categories
Funding
- National Natural Science Foundation of China [21205094]
- NFFTBS [J1103311, J1210057]
- New Faculty Startup Funds of Northwest University in Shaanxi Province [PR12011]
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This paper describes a low-cost, sensitive, visual and rapid immunochromatographic assay method on cotton thread for carcinoembryonic antigen (CEA) detection by using novel carbon nanotube/gold nanoparticles (CNT/GNPs) nanocomposite reporter probe. CEA, a lung cancer protein biomarker, was used as analyte to demonstrate the principle of the immunochromatographic assay on cotton thread biosensor. In the presence of target CEA, the decreasing aggregation amount of CNT/GNPs nanocomposite reporter probes on the test zone induced directly readout by naked eye. Meanwhile, quantitative detection could be performed conveniently with a commercial available scanner. The performance with respect to sensitivity of the method was greatly improved by 2-3 magnitudes comparing with traditional gold nanoparticles (GNPs) or carbon nanotubes (CNTs) as reporter probe. Under optimal conditions, the biosensor was capable of detecting 2.32 ng/ mL CEA (S/N >= 3) which is sensitive enough for clinical diagnosis. These results indicated the novel CNT/GNPs nanocomposite reporter probe based immunochromatographic assay on cotton thread is particularly suitable for point-of-care (POC) diagnostics in resource-limited regions. (C) 2017 Elsevier B.V. All rights reserved.
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