Journal
ELECTROCHIMICA ACTA
Volume 236, Issue -, Pages 319-327Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2017.03.161
Keywords
3D graphene; MC-LR; biosensor; electrochemical detection; nanomaterials
Categories
Funding
- Ohio Sea Grant, USA [R/PS-052-PD]
- International Scientific Cooperation Project of Changzhou Science and Technology Bureau, China [CJ20140718]
- Australian Endeavour Fellowship Program
- University of South Australia Early Career Researcher Travel Grant Scheme
- Prince Sultan Bin Abdulaziz International Prize for Water-Alternative Water Resources Prize
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Recent advances in graphene synthesis and understanding of properties have led to enormous applications in a variety of areas. Graphene and its unique electrical properties can favor electrochemical biosensor applications for aqueous toxin monitoring. Graphene-based biosensors can be used as an alternative to time-consuming, expensive and non-portable conventional methods of analysis involved in water quality monitoring and assessment. In this work, we showcased a three-dimensional (3D) graphene-based biosensor for microcystin-LR (MC-LR) detection and quantification. We report the efficient functionalization and immobilization of microcystin-LR and its antibodies on the facile synthesized CVD 3D graphene. The modified graphene electrodes were characterized by X-ray photoelectron spectroscopy and micro-Raman spectroscopy. Cyclic voltammetry and electrochemical impedance spectroscopy were used to electrochemically characterize the biochemical events on the electrodes. Specifically, as-prepared 3D graphene-based biosensors can detect MC-LR with remarkable sensitivity due to its macro-porous structure and large surface area, and high conductivity. A very good linear correlation of the electron-transfer resistance (R-2 = 0.93) was achieved over 0.05 and 20 mu g/L MC-LR concentration range. Also, a detection limit of 0.05 mu g/L was accomplished, which is much lower than the World Health Organization (WHO) provisional guideline limit of MC-LR concentration (i.e. 1 mu g/L) in drinking water. (C) 2017 Elsevier Ltd. All rights reserved.
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