Journal
ANALYTICA CHIMICA ACTA
Volume 967, Issue -, Pages 12-32Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2017.01.060
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Funding
- Czech Science Foundation [P206/12/0398]
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Hydrophilic interaction chromatography (HILIC) is a rapidly developing liquid chromatography mode suitable for separation of strongly or moderately polar samples on polar columns in highly organic mixed mobile phases. In the past five years many new types of columns appeared based on silica gel, organic polymers and other types of supports. The present work reviews the new additions to their family. Further, the progress in the understanding of theoretical principles of the HILIC mode, especially of the role of the mobile phase, and of the adsorbed water are addressed. The amount of adsorbed water strongly depends on the type of the polar column and may be used to distinguish between conventional HILIC and aqueous normal phase chromatography (ANP). Further, sample structural effects on the retention are treated in terms of the Linear Solvation Energy Relationship model adopted for partially ionized polar compounds. The impact of dual HILIC-reversed phase (RP) mechanism on a possible increase of the applications of some polar columns is discussed. Attention is paid also to the increasing role of the HILIC mode in various two-dimensional LC techniques and the related mobile phase compatibility problems. (C) 2017 Elsevier B. V. All rights reserved.
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