Journal
BIOSENSORS & BIOELECTRONICS
Volume 91, Issue -, Pages 538-544Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.01.009
Keywords
Upconversion nanoparticles; Ochratoxin A; Graphene oxide; Luminescence resonance energy transfer
Categories
Funding
- Key Research and Development Program of Jiangsu Province [BE2016306]
- National Natural Science Foundation of China [21375049, 31401576]
- China Postdoctoral Science Foundation [2015M580402, 2016T90430]
- Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province
- [BK20140155]
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We developed an ultrasensitive luminescence resonance energy transfer (LRET) aptasensor for Ochratoxin A (OTA) detection, using core/shell upconversion nanoparticles (CS-UCNPs) as luminophores. The OTA aptamer was tagged to CS-UCNPs as energy donor and graphene oxide (GO) acted as energy acceptor. The pi-pi stacking interaction between the aptamer and GO brought CS-UCNPs and GO in close proximity hence initiated the LRET process resulting in quenching of CS-UCNPs luminescence. A linear calibration was obtained between the luminescence intensity and the logarithm of OTA concentration in the range from 0.001 ng mL(-1) to 250 ng mL(-1), with a detection limit of 0.001 ngmL(-1). The aptasensor showed good specificity towards OTA in beer samples. The ultrahigh sensitivity and pronounced robustness in beer sample matrix suggested promising prospect of the aptasensor inpractical applications.
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