Journal
BIOSENSORS & BIOELECTRONICS
Volume 91, Issue -, Pages 863-869Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.01.053
Keywords
Amphiphilic pillar[5]arene; Host guest interaction; Reduced graphene.mdde; Fluorescence sensing; Acetaminophen
Categories
Funding
- National Natural Science Foundation of China [21565029]
- Program for Excellent Young Talents, Yunnan University [WX069051]
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A sensitive and selective fluorescence approach based on a competitive host guest interaction between amphiphilic pillar[5]arene (amPA5) and signal probe (acridine orange, AO)/target molecule (acetaminophen, AP) was developed by using amPA5 functionalized reduced graphene oxide (amPA5-RGO) as a receptor. Due to the host guest interaction, AO and AP molecules both can enter into the hydrophobic inner cavity of amPA5 that could form a complex of 1:1 guest host with amPA5 according to the size of molecules and the cavity of amPA5, but the AP interacts more strongly with amPA5 than with AO, so it can detect AP by the host-guest competition. The low detection limit of 0.05 mu M (S/N=3) and a linear response range of 0.1-4.0 mu M and 4.0-32 mu M for AP was obtained by using this method. It had lower detection limit and wider linear range than other methods, therefore, it was successfully utilized to detect AP in serum samples, and exhibited a promising application in practice. The molecular docking studies indicated that the major driving forces for the formation of the inclusion complex of AP and amPA5 are hydrogen bonding, pi-pi interactions, and hydrophobic interactions.
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