Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 114, Issue 6, Pages 1201-1207Publisher
WILEY
DOI: 10.1002/bit.26252
Keywords
cellulosic ethanol; crystalline cellulose; cell-surface display; cellulase ratio; Saccharomyces cerevisiae
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Funding
- Special Coordination Funds
- National Research Foundation of South Africa
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science (JSPS)
- NRF of South Africa
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Crystalline cellulose is one of the major contributors to the recalcitrance of lignocellulose to degradation, necessitating high dosages of cellulase to digest, thereby impeding the economic feasibility of cellulosic biofuels. Several recombinant cellulolytic yeast strains have been developed to reduce the cost of enzyme addition, but few of these strains are able to efficiently degrade crystalline cellulose due to their low cellulolytic activities. Here, by combining the cellulase ratio optimization with a novel screening strategy, we successfully improved the cellulolytic activity of a Saccharomyces cerevisiae strain displaying four different synergistic cellulases on the cell surface. The optimized strain exhibited an ethanol yield from Avicel of 57% of the theoretical maximum, and a 60% increase of ethanol titer from rice straw. To our knowledge, this work is the first optimization of the degradation of crystalline cellulose by tuning the cellulase ratio in a cellulase cell-surface display system. This work provides key insights in engineering the cellulase cocktail in a consolidated bioprocessing yeast strain. Biotechnol. Bioeng. 2017;114: 1201-1207. (c) 2017 Wiley Periodicals, Inc.
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