Journal
BLOOD
Volume 129, Issue 18, Pages 2507-2518Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2016-08-737239
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Funding
- Ligue Nationale Contre le Cancer (Equipe Labellisee and Carte d'identite des Tumeurs program)
- Institut National du Cancer [INCA AAP PLBIO-14-40]
- FP7 Marie Curie Initial Training Network [ITN 289720 Stroma]
- Ligue Nationale Contre le Cancer
- Mildred-Scheel-Cancer Foundation
- Michael Smith Foundation for Health Research
- Lymphoma Canada
- Medical Research Council [G0601156, MR/M009157/1] Funding Source: researchfish
- National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) [NC/K000527/1] Funding Source: researchfish
- Versus Arthritis [21236] Funding Source: researchfish
- MRC [G0601156, MR/M009157/1] Funding Source: UKRI
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Follicular lymphoma (FL) is the most frequent indolent lymphoma and is characterized by the accumulation of germinal center-derived malignant B cells engaged in a bidirectional crosstalk with their supportive microenvironment in invaded lymph nodes (LNs) and bone marrow (BM). T follicular helper (T-FH) cells and infiltrating stromal cells have been shown to favor FL B-cell growth, but the mechanisms of their protumoral effect and how the LN/BM microenvironment is converted into a lymphoma-permissive cell niche remain poorly understood. We demonstrated here that FL-infiltrating LN and BM stromal cells overexpressed CXCL12 in situ. Interleukin-4 high (IL-4(hi)) FL-T-FH cells, unlike FL B cells themselves, triggered CXCL12 upregulation in human stromal cell precursors. In agreement, expression of CXCL12 was associated with IL-4 expression and signaling within the FL BM and LN niches. This IL-4/CXCL12 axis was amplified in activated lymphoid stromal cells as shown in our in vitro model of human lymphoid stroma differentiation and in an inducible mouse model of ectopic lymphoid organ formation. Finally, CXCL12 triggered primary FL B-cell activation, migration, and adhesion, a process antagonized by BTK and PI3K inhibitors. These data identified the IL-4/CXCL12 loop as a previously unrecognized pathway involved in lymphoid stroma polarization and as a potential therapeutic target in FL patients.
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