Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 16, Pages 14162-14170Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b02523
Keywords
PEDOT; influenza virus; glycosylation; recognition; impedance; QCM; potentiometry
Funding
- MEXT of Japan [26107705]
- Futaba Electronics Foundation
- ImPACT Miyata program from the JST agency
- Grants-in-Aid for Scientific Research [26107705] Funding Source: KAKEN
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Conducting polymers are good candidates for biosensor applications when molecular recognition element is imparted. We developed trisaccharide-grafted conducting polymers for label-free detection of the human influenza A virus (H1N1) with high sensitivity and specificity. A 3,4-ethylenedioxythiophene (EDOT) derivative bearing an oxylamine moiety was electrochemically copolymerized with EDOT. The obtained film was characterized by cyclic voltammetry, X-ray photoelectron spectroscopy, scanning electron microscopy, stylus surface profilometer, and AC-impedance spectroscopy. The trisaccharides comprising Sia-alpha 2,6'-Gal-Glu (2,6-sialyllactose) or Sia-alpha 2,3'-Gal-Glu (2,3sialyllactose) were covalently introduced to the side chain of the conducting polymers as a ligand for viral recognition. Immobilization of sialyllactose was confirmed by quartz crystal microbalance (QCM) and water contact angle measurements. Specific interaction of 2,6-sialyllactose with hemagglutinin in the envelope of the human influenza A virus (H1N1) was detected by QCM and potentiometry with enhanced sensitivity by 2 orders of magnitude when compared with that of commercially available kits. The developed conducting polymers possessing specific virus recognition are a good candidate material for wearable monitoring and point-of-care testing because of their processability and mass productivity in combination with printing
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