4.7 Article

Highly Luminescent Folate-Functionalized Au22 Nanoclusters for Bioimaging

Journal

ADVANCED HEALTHCARE MATERIALS
Volume 6, Issue 16, Pages -

Publisher

WILEY
DOI: 10.1002/adhm.201700203

Keywords

Au-22 nanoclusters; bioimaging; folate; luminescent probes

Funding

  1. Korea CCS RD Center (KCRC) [NRF-2014M1A8A1074219]
  2. NRF [NRF-2014R1A2A1A11051032, 2009-0093823]
  3. Yonsei University Future-leading Research Initiative

Ask authors/readers for more resources

Gold nanoclusters are emerging as new materials for biomedical applications because of promises offered by their ultrasmall size and excellent biocompatibility. Here, the synthesis and optical and biological characterizations of a highly luminescent folate-functionalized Au-22 cluster (Au-22-FA) are reported. The Au-22-FA clusters are synthesized by functionalizing the surface of Au-22(SG)(18) clusters, where SG is glutathione, with benzyl chloroformate and folate. The functionalized clusters are highly water-soluble and exhibit remarkably bright luminescence with a quantum yield of 42%, significantly higher than any other water-soluble gold clusters protected with thiolate ligands. The folate groups conjugated to the gold cluster give rise to additional luminescence enhancement by energy transfer sensitization. The brightness of Au-22-FA is found to be 4.77 mM(-1) cm(-1), nearly 8-fold brighter than that of Au-22(SG)(18). Further biological characterizations have revealed that the Au-22-FA clusters are well-suited for bioimaging. The Au-22-FA clusters exhibit excellent photostability and low toxicity; nearly 80% cell viability at 1000 ppm of the cluster. Additionally, the Au-22-FA clusters show target specificity to folate-receptor positive cells. Finally, the time-course in vivo luminescence images of intravenous-injected mice show that the Au-22-FA clusters are renal-clearable, leaving only 8% of them remained in the body after 24 h post-injection.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available