Journal
ADVANCED HEALTHCARE MATERIALS
Volume 6, Issue 9, Pages -Publisher
WILEY
DOI: 10.1002/adhm.201601439
Keywords
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) Japan [23651142]
- Japan Society for the Promotion of Science (JSPS)
- JSPS
- Grants-in-Aid for Scientific Research [15KK0246, 16K01402, 23651142] Funding Source: KAKEN
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A novel shape-memory cell culture platform has been designed that is capable of simultaneously tuning surface topography and dimensionality to manipulate cell alignment. By crosslinking poly(epsilon-caprolactone) (PCL) macro-monomers of precisely designed nanoarchitectures, a shape-memory PCL with switching temperature near body temperature is successfully prepared. The temporary strain-fixed PCLs are prepared by processing through heating, stretching, and cooling about the switching temperature. Temporary nano-wrinkles are also formed spontaneously during the strain-fixing process with magnitudes that are dependent on the applied strain. The surface features completely transform from wrinkled to smooth upon shape-memory activation over a narrow temperature range. Shape-memory activation also triggers dimensional deformation in an initial fixed strain-dependent manner. A dynamic cell-orienting study demonstrates that surface topographical changes play a dominant role in cell alignment for samples with lower fixed strain, while dimensional changes play a dominant role in cell alignment for samples with higher fixed strain. The proposed shape-memory cell culture platform will become a powerful tool to investigate the effects of spatiotemporally presented mechanostructural stimuli on cell fate.
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