Journal
ADVANCED HEALTHCARE MATERIALS
Volume 6, Issue 20, Pages -Publisher
WILEY
DOI: 10.1002/adhm.201700642
Keywords
cationic polymers; initiated chemical vapor deposition (iCVD); nucleic acid extraction; pathogen detection
Funding
- Advanced Biomass R&D Center of Global Frontier Project - Ministry of Science, ICT and Future Planning [ABC-2010-0029728]
- BioNano Health-Guard Research Center - Ministry of Science, ICT & Future Planning (MSIP) of Korea [H-GUARD_2013M3A6B2078945]
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning (MSIP) of Korea [NRF-2013M3A2A1073991]
- Ministry of Science & ICT (MSIT), Republic of Korea [NNFC-17-01] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2014M3A6B2060302, 2010-0029728, 2013M3A2A1073991] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Rapid and convenient isolation of nucleic acids (NAs) from cell lysate plays a key role for onsite gene expression analysis. Here, this study achieves one-step and efficient capture of NA directly from cell lysate by developing a cationic surface-modified mesh filter (SMF). By depositing cationic polymer via vapor-phase deposition process, strong charge interaction is introduced on the surface of the SMF to capture the negatively charged NAs. The NA capturing capability of SMF is confirmed by X-ray photoelectron spectroscopy, fluorescent microscopy, and zeta potential measurement. In addition, the genomic DNAs of Escherichia Coli O157:H7 can be extracted by the SMF from artificially infected food, and fluorescent signal is observed on the surface of SMF after amplification of target gene. The proposed SMF is able to provide a more simplified, convenient, and fast extraction method and can be applied to the fields of food safety testing, clinical diagnosis, or environmental pollutant monitoring.
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