Journal
BIORESOURCE TECHNOLOGY
Volume 229, Issue -, Pages 190-195Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.01.004
Keywords
Lignocellulose; Pretreatment; Ionic liquid; Enzymatic hydrolysis; Inhibition; Deactivation
Funding
- New National Excellence Program of the Ministry of Human Capacities [UNKP-2016-4-04]
- National Research, Development and Innovation Office (Hungary) [PD 115640]
- [2013-0008]
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The aim of this work was to study the inhibition and deactivation of commercial enzyme cocktail (Cellic (R) Htec2) in the presence of [bmim][Cl] ionic liquid employing model cellulosic substrate, carboxymethyl cellulose (CMC). It turned out from the experiments - relying on enzyme kinetics approach - that [bmim][Cl] could act as a competitive inhibitor. Furthermore, depending on the process conditions i.e. contact of enzyme solution with high concentration [bmim][Cl], severe biocatalyst inactivation should be also taken into account as a potential risk during the enzymatic cellulose hydrolysis even in as short process times as few minutes. (C) 2017 Elsevier Ltd. All rights reserved.
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