Journal
ACS SYNTHETIC BIOLOGY
Volume 6, Issue 12, Pages 2293-2301Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00219
Keywords
Pseudomonas aeruginosa; cystic fibrosis; quorum sensing; cell-free synthetic biology; biosensor
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Funding
- EPSRC studentship
- CF Trust studentship (CFT SRC1)
- MRC studentship
- EPSRC IKC [EP/L011573/1]
- Cystic Fibrosis Trust
- BBSRC [BB/M025632/1, BB/L007959/1] Funding Source: UKRI
- EPSRC [EP/N023854/1, EP/L011573/1] Funding Source: UKRI
- MRC [MR/P028225/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L007959/1, BB/M025632/1] Funding Source: researchfish
- Cystic Fibrosis Trust [SRC001] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/N023854/1, 1812318, EP/L011573/1] Funding Source: researchfish
- Medical Research Council [MR/P028225/1] Funding Source: researchfish
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Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, we report that a modular DNA-encoded biosensor in cell-free protein expression systems can be used to measure a bacterial biomarker of Pseudomonas aeruginosa infection from human sputum samples. By optimizing the cell-free system and sample extraction, we demonstrate that the quorum sensing molecule 3-oxo-C12-HSL in sputum samples from cystic fibrosis lungs can be quantitatively measured at nanomolar levels using our cell-free biosensor system, and is comparable to LC-MS measurements of the same samples. This study further illustrates the potential of modular cell-free biosensors as rapid, low-cost detection assays that can inform clinical practice.
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