Journal
ACS SYNTHETIC BIOLOGY
Volume 7, Issue 2, Pages 339-346Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00306
Keywords
communication; cell-free expression; lipid vesicles; proteinosomes; genetic switches; enzyme cascade; alpha-hemolysin
Categories
Funding
- BBSRC/EPSRC [BB/L01386X/1]
- BrisSynBio (a BBSRC/EPSRC synthetic Biology research centre)
- MaxSynBio consortium (Federal Ministry of Education and Research of Germany)
- MaxSynBio consortium (Max Planck Society)
- MPG
- Erasmus
- Armenise-Harvard Foundation
- Simons Foundation [290358]
- EPSRC Early Career Fellowship [EP/K026720/1]
- Biotechnology and Biological Sciences Research Council [BB/L01386X/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/N026586/1, EP/K026720/1] Funding Source: researchfish
- BBSRC [BB/L01386X/1] Funding Source: UKRI
- EPSRC [EP/K026720/1, EP/N026586/1] Funding Source: UKRI
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A gene-directed chemical communication pathway between synthetic protocell signaling transmitters (lipid vesicles) and receivers (proteinosomes) was designed, built and tested using a bottom-up modular approach comprising small molecule transcriptional control, cell-free gene expression, porin-directed efflux, substrate signaling, and enzyme cascade-mediated processing.
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