Journal
ACS SYNTHETIC BIOLOGY
Volume 6, Issue 7, Pages 1140-1149Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.6b00271
Keywords
DNA origami; sequence design and optimization; nanotechnology; synthetic biology
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Funding
- EPSRC [EP/J004111/2, EP/L001489/2, EP/N031962/1]
- Engineering and Physical Sciences Research Council [EP/L001489/2, EP/N031962/1, EP/J004111/2] Funding Source: researchfish
- EPSRC [EP/L001489/2, EP/J004111/2, EP/N031962/1] Funding Source: UKRI
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Nanotechnology and synthetic biology are rapidly converging, with DNA origami being one of the leading bridging technologies. DNA origami was shown to work well in a wide array of biotic environments. However, the large majority of extant DNA origami scaffolds utilize bacteriophages or plasmid sequences thus severely limiting its future applicability as a bio-orthogonal nanotechnology platform. In this paper we present the design of biologically inert (i.e., bio-orthogonal) origami scaffolds. The synthetic scaffolds have the additional advantage of being uniquely addressable (unlike biologically derived ones) and hence are better optimized for high-yield folding. We demonstrate our fully synthetic scaffold design with both DNA and RNA origamis uniquely addressable origami scaffolds. and describe a protocol to produce these bio-orthogonal and
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