Journal
ACS SYNTHETIC BIOLOGY
Volume 7, Issue 1, Pages 30-37Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00277
Keywords
aptamer; strand displacement; dynamics; synthetic biology
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Funding
- National Science Foundation [CMMI-1266402]
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-SC0010595]
- U.S. Department of Energy (DOE) [DE-SC0010595] Funding Source: U.S. Department of Energy (DOE)
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Nucleic acid aptamers are an expandable toolkit of sensors and regulators. To employ aptamer regulators within nonequilibrium molecular networks, the aptamer-ligand interactions should be tunable over time, so that functions within a given system can be activated or suppressed on demand. This is accomplished through complementary sequences to aptamers, which achieve programmable aptamer-ligand dissociation by displacing the aptamer from the ligand. We demonstrate the effectiveness of our simple approach on light-up aptamers as well as on aptamers inhibiting viral RNA polymerases, dynamically controlling the functionality of the aptamer-ligand complex. Mathematical models allow us to obtain estimates for the aptamer displacement kinetics. Our results suggest that aptamers, paired with their complement, could be used to build dynamic nucleic acid networks with direct control over a variety of aptamer-controllable enzymes and their downstream pathways.
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