Journal
ACS SYNTHETIC BIOLOGY
Volume 6, Issue 9, Pages 1642-1649Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00113
Keywords
RNA editing photocontrol; photocaging; protein localization; optogenetics; epitranscriptomics; gene regulation
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Funding
- University of Tubingen
- Deutsche Forschungsgemeinschaft [STA1053/3-2, STA1053/4-1, STA1053/7-1]
- European Research Council (ERC) under the European Union's Horizon research and innovation program [647328]
- European Research Council (ERC) [647328] Funding Source: European Research Council (ERC)
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Site directed RNA editing is an engineered tool for the posttranscriptional manipulation of RNA and proteins. Here, we demonstrate the inclusion of additional N-and C-terminal protein domains in an RNA editing-dependent manner to switch between protein isoforms in mammalian cell culture. By inclusion of localization signals, a switch of the subcellular protein localization was achieved. This included the shift from the cytoplasm to the outer-membrane, which typically is inaccessible at the protein-level. Furthermore, the strategy allows to implement photocaging to achieve spatiotemporal control of isoform switching. The strategy does not require substantial genetic engineering, and might well complement current optogenetic and optochemical approaches.
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