Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 9, Pages 5003-5007Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b00329
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Funding
- National Natural Science Foundation of China [21235003, 21327902, 81672570]
- Science Foundation of Jiangsu Province, China [BM2015023]
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The interaction between protein and DNA elements controls a variety of functions of genomes. The development of a convenient and cost-effective method for investigating the sequence specificity of DNA-binding proteins represents an important challenge. In response, we have introduced an electrochemical assay in this work for specific and sensitive analysis of interaction between protein and nucleic acid in nucleic extracts, based on the protein-induced distinctive motion behavior of DNA deoxyribozyme (DNAzyme) on an electrode surface. As a proof of principle, we have also presented assays for the rapid, sensitive, and selective detection of three transcription factors (NF-kappa B, SP6 RNA polymerase, and HNF-4 alpha), as well as the analysis of binding affinity of the mutated protein-binding sequence, and even screening of the binding sequence of HNF-4 alpha protein in vitro. This work may open new opportunity for in-depth profiling of the sequence specificity of DNA-binding proteins and study of nucleotide polymorphisms in known protein-binding sites.
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