Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 88, Issue 1, Pages 69-74Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2017.01.024
Keywords
Uropathogenic Escherichia coli; ST73; Virulence factors; Urinary tract infection
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Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) of Brazil
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) of Brazil
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ) of Brazil
- Fogarty International Program in Global infectious Diseases of the National Institute of Health of the US [TW006563]
- NIH S10 Instrumentation Grants [S10RR029668, S10RR027303]
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Escherichia coli clones ST131, ST69, ST95, and ST73 are frequent causes of urinary tract infections (UTI) and bloodstream infections. Specific clones and virulence profiles of E. coli causing UTI in men has been rarely described. The aim of this study was to characterize patient and clonal characteristics of community-acquired UTI caused by E. coli in men (n = 12) and women (n = 127) in Rio de Janeiro, Brazil, complementing a previous work. We characterized isolates in phylogenetic groups, ERIC2-PCR and PFGE types, MLST, genome similarity and virulence gene-profiles. UTI from men were more frequently caused by phylogenetic group B2 isolates (83% versus 42%, respectively, P = 0.01), a group with significantly higher virulence scores compared with women. ST73 was the predominant clone in men (50%) and the second most frequent in women (12%), with the highest virulence score (mean and median = 9) among other clones. ST73 gnomes formed at least six clusters. E. coli from men carried significantly higher numbers of virulence genes, such as sfa/focDE (67% versus 27%), hlyA (58% versus 24%), cnf 1 (58% versus 16%), fyuA (100% versus 82%) and MalX (92% versus 44%), compared with isolates from women. These data suggest the predominance and spread of ST73 isolates likely relates to an abundance of virulence determinants. (C) 2017 Elsevier Inc. All rights reserved.
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