Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 73, Issue -, Pages 88-96Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2017.03.015
Keywords
Eleginops maclovinus; Ferritin; Gene expression; Iron; Notothenioid
Categories
Funding
- Fondap-Incar [15110027]
- DID
- UACH
- Fondap-Ideal Grant [15150003]
- Fondecyt [1160877]
- Comision Nacional de Investigacion Cientifica y Tecnologica
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Ferritin is a major iron storage protein essential not only in the infectious process, but also in any circumstance generating oxidative stress. In this study, the cDNA coding sequence of ferritin-H was obtained from the sub-Antarctic Notothenioid fish Eleginops maclovinus through transcriptomic analysis of the head kidney. This sequence contained a 534 bp open reading frame that coded for a 177 amino acid protein with a molecular weight of 20,786.2 Da and a theoretical pI of 5.56. The protein displayed a region of iron putative response elements in the 5'UTR, two putative ferritin iron-binding region signatures, and seven characteristic amino acids with ferroxidase functions. Phylogenetic analysis related this sequence to ferritin-H sequences of other Antarctic Notothenioid fish, sharing 96.61% similarity. Constitutive gene expression analysis in different organs revealed increased ferritin-H gene expression in the gills, spleen, muscle, and liver. After infection with two bacterial strains of Piscirickettsia salmonis (LF-89 and Austral-005), ferritin-H was differentially expressed depending on bacterial strain and tissue. This study provides relevant information towards understanding the iron metabolism of a sub-Antarctic Notothenioid fish. (C) 2017 Elsevier Ltd. All rights reserved.
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