Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 486, Issue 4, Pages 1090-1096Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2017.03.167
Keywords
Metabolic labeling; Sialylation; ST6GAL1; Colorectal cancer
Categories
Funding
- National Natural Science Foundation of China [31370806, 31170771, 31600643, 31670807]
- National Basic Research Program of China [2012CB822103, 2011CB910603]
- Shanghai Municipal Commission of Health and Family Planning Program [201640030]
- Shanghai Jiao Tong University Interdiscipline with Medicine Program [YG2013MS26]
- China Postdoctoral Science Foundation [2016M600310]
- Grants-in-Aid for Scientific Research [15H04354] Funding Source: KAKEN
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Elevated expression of beta-galactoside alpha 2,6-sialyltranferase 1 (ST6GAL1) has been observed in colorectal cancer (CRC) and demonstrated to be important for its tumorigenesis. Here, we found that ST6GAL1 expression was significantly higher in non-metastatic tumors (stage I and II) than that in metastatic tumors (stage III and IV) using 62 pair-matched tumor/normal tissues. To elucidate the molecular mechanisms of how ST6GAL1 affected the CRC progression, we performed a global identification of the substrates of ST6GAL1 in the colon adenocarcinoma cell line SW480. A total of 318 membrane proteins were identified differentially affected by ST6GAL1 overexpression using metabolic labeling and proteomic analysis. Subsequent bioinformatic analysis revealed a list of potential substrates that might mediate the different functions of ST6GAL1 in CRC including cell movement, cell death and survival. Taken together, these results indicate a dynamic change in the expression of ST6GAL1 during the CRC progression and provide a list of sialylated proteins potentially relevant to the different functions of ST6GAL1 in CRC. (C) 2017 Elsevier Inc. All rights reserved.
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