Journal
VIRULENCE
Volume 9, Issue 1, Pages 197-207Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/21505594.2017.1384895
Keywords
Vibrio parahaemolyticus; nudix hydrolase; MutT; Galleria mellonella
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Funding
- Wellcome Trust Institutional Strategic Support Fund [WT097835MF]
- Wellcome Trust Multi User Equipment Award [WT097835MF]
- Medical Research Council Clinical Infrastructure Funding [MR/M008924/1]
- Biotechnology and Biological Sciences Research Council (BBSRC) funding [BB/N016513/1]
- BBSRC [BB/N016513/1] Funding Source: UKRI
- MRC [MR/M008924/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/N016513/1] Funding Source: researchfish
- Medical Research Council [MR/M008924/1] Funding Source: researchfish
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Non-toxigenic V. parahaemolyticus isolates (tdh(-)/trh(-)/T3SS2(-)) have recently been isolated from patients with gastroenteritis. In this study we report that the larvae of the wax moth (Galleria mellonella) are susceptible to infection by toxigenic or non-toxigenic clinical isolates of V. parahaemolyticus. In comparison larvae inoculated with environmental isolates of V. parahaemolyticus did not succumb to disease. Whole genome sequencing of clinical non-toxigenic isolates revealed the presence of a gene encoding a nudix hydrolase, identified as mutT. A V. parahaemolyticus mutT mutant was unable to kill G. mellonella at 24 h post inoculation, indicating a role of this gene in virulence. Our findings show that G. mellonella is a valuable model for investigating screening of possible virulence genes of V. parahaemolyticus and can provide new insights into mechanisms of virulence of atypical non-toxigenic V. parahaemolyticus. These findings will allow improved genetic tests for the identification of pathogenic V. parahaemolyticus to be developed and will have a significant impact for the scientific community.
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