Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 409, Issue 14, Pages 3587-3596Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-017-0300-3
Keywords
Column switching; Liquid chromatography-tandem mass spectrometry; Endocannabinoids; Plasma samples
Funding
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2015/07619-1, 2016/13639-8]
- CNPq Brazil (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [26/2014, 466805/2014-4]
Ask authors/readers for more resources
This study reports a fast, sensitive, and selective column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to determine the endocannabinoids (eCBs), anandamide (AEA), and 2-arachidonoylglycerol (2-AG) in plasma samples. This bidimensional system used a restricted access media column (RP-8 ADS, 25 mm x 4 mm x 25 mu M) in the first dimension and a core-shell Kinetex C18 (100 mm x 2, 1.7 mm x 1 mu M) column in the second dimension, followed by detection in a mass spectrometer triple quadrupole (multiple reactions monitoring mode) operating in the positive mode. RP-8 ADS was used for trace enrichment of eCBs (reverse phase partitioning) and macromolecular matrix size exclusion; the core-shell column was used for the chromatographic separation. The column switching UHPLC-MS/MS method presented a linear range spanning from 0.1 ng mL(-1) (LOQ) to 6 ng mL(-1) for AEA and from 0.04 ng mL(-1) (LOQ) to 10 ng mL(-1) for 2-AG. Excluding the LLOQ values, the precision assays provided coefficients of variation lower than 8% and accuracy with relative standard error values lower than 14%. Neither carryover nor matrix effects were detected. This high-throughput column switching method compared to conventional methods is time saving as it involves fewer steps, consumes less solvent, and presents lower LLOQ. The column switching UHPLC-MS/MS method was successfully applied to determine AEA and 2-AG in plasma samples obtained from Alzheimer's disease patients.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available