Journal
THERANOSTICS
Volume 7, Issue 18, Pages 4424-4444Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.18832
Keywords
One-Pot Synthesis; Thermally Sensitive; Gold Nanoparticle; Pluronic-b-poly(L-lysine); Drug Delivery; Chemo-Photothermal
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Funding
- National Natural Science Foundation of China [81472841, 81572999, 81502692, 81602718, 81771839, 81773272]
- State Key Laboratory of Oncogenes and Related Genes [91-17-02]
- Three-year Action Plan for Further Development of Traditional Chinese Medicine of Shanghai [ZY3-CCCX-3-3032]
- Shanghai Municipal Health and Family Planning Commission Foundation [201440015]
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Gold nanoparticle-coated Pluronic-b-poly(L-lysine) nanoparticles (Pluronic-PLL@Au NPs) were synthesized via an easy one-step method and employed as carriers for the delivery of paclitaxel (PTX) in chemo-photothermal therapy, in which Pluronic-PLL acts as the reductant for the formation of AuNPs without the need for an additional reducing agent. Methods: The deposition of AuNPs on the surface of Pluronic-PLL micelles and the thermal response of the system were followed via ultraviolet-visible spectroscopy and dynamic light scattering. Calcein-AM and MTT assays were used to study the cell viability of MDA-MB-231 cells treated with PTX-loaded Pluronic-PLL@Au NPs, and we then irradiated the cells with NIR light. Results: An obvious temperature response was observed for the Pluronic-PLL@Au NPs. Blood compatibility and in vitro cytotoxicity assays confirmed that the Pluronic-PLL@Au NPs have excellent biocompatibility. Compared to Taxol, the PTX-loaded Pluronic-PLL@Au NPs exhibited higher cytotoxicity in MDA-MB-231 cells. All of these results and confocal laser scanning microscopy analysis results suggest that Pluronic-PLL@Au NPs greatly enhance the cellular uptake efficiency of the drug. Conclusion: As confirmed by in vitro and in vivo studies, the combination of chemotherapy and photothermal therapy can cause more damage than chemo-or photothermal therapy did alone, demonstrating the synergistic effect of chemo-photothermal treatment. Thus, the as-prepared Pluronic-PLL@Au NPs are promising for chemo-photothermal therapy.
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