QUANITITATIVE ANALYSIS OF PYRETHRINS FROM CHRYSANTHEMUM CINERARIIAEFOLIUM AND CHRYSANTHEMUM LEUCANTEUM PETALS

Pyrethrins content was determined for two chrysanthemum species namely: Chrysanthemum cinerariifolium and Chrysanthemum leucantheum In order to determine the most efficient solvent for pyrethrins extraction three solvents were used: petroleum ether (I), a mixture of petroleum ether, ethanol and acetone (1: 1: 1) (II) and methanol (III) Spectrophotometric method used for determination of pyrethrins is based on pyrethrins colour reaction with 2,4- dinitrophenylhydrazine followed by measurements of resulting compound at 377 nm wavelength. Pyrethrin content was determined comparing to alpha-cypermethrin calibration curve. According to the obtained results for the analysed chrysanthemum extracts the most efficient solvent for pyrethrins extraction was methanol and the highest pyrethrins content was found in Chrysanthemum cinerariifolium petals.