Journal
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Volume 93, Issue -, Pages 492-500Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejmech.2015.02.025
Keywords
Loureirin A; Cochinchinenin C; Human serum albumin; Fluorescence quenching; Molecular docking
Categories
Funding
- National Key Technology Support Program of China [2013BAD12B06]
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The interactions of loureirin A (LA) and cochinchinenin C (CC) with human serum albumin (HSA) under simulated physiological conditions (pH = 7.4) have been studied with fluorescence, UV-vis absorption spectroscopic method and molecular docking technique. The results indicated that there was a synergistic interaction between LA and CC, and the fluorescence quenching of HSA by LA (or CC) was a combined quenching procedure (dynamic and static quenching). At low compound concentrations, the quenching constants K-SV of CC was larger than that of LA, which meant the CC efficacy may be better than that of LA. The negative Delta H and Delta S values suggested hydrogen bonds and van der Waals forces played the major role in the binding of LA (or CC) to HSA. The efficiency of energy transfer and distance between the compounds and HSA was calculated. Moreover, the results of synchronous and three-dimensional fluorescence demonstrated that the HSA microenvironment was changed in the presence of LA (or CC). Finally, the binding of LA (or CC) to HSA was modeled by molecular docking, which is in good accordance with the experimental studies. (C) 2015 Elsevier Masson SAS. All rights reserved.
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