Journal
CARBOHYDRATE POLYMERS
Volume 165, Issue -, Pages 189-196Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.02.032
Keywords
Sulfated polysaccharide from Porphyra haitanensis; Immunomodulatory; RAW264.7; T lymphocytes; Dendritic cell; Regulatory T cell
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Funding
- National Natural Scientific Foundation of China [U1405214]
- Scientific Foundation of Fujian Province [2014N0014]
- Marine Scientific Research Special Foundation for Public Sector Program [201505026-03]
- Xiamen South Ocean Research Center Project [201405220001]
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The immunoregulatory activity of sulfated polysaccharide from Porphyra haitanensis (PHPS) was investigated in a RAW264.7 macrophages cell model and a BALB/c murine model. The subpopulation of dendritic cells (DCs) and regulatory T cells (Tregs) from PHPS-treated mice splenocytes were also measured by flow cytometry. Consistent with previous reports, we showed that PHPS increased the phagocytosis of RAW264.7 macrophages, and enhanced the secretion of interleukin (IL)-6, IL-10 and tumor necrosis factor-alpha (TNF-alpha). Meanwhile, PHPS,induced the production of nitric oxide via the Jun N-terminal kinase (JNK) and the Janus kinase (JAK2) signaling pathways in RAW264.7 macrophages. Furthermore, PHPS promoted the proliferation of mice lymphocytes, inducing the generation of TNF-a and IL-10 in vivo, as well as the subpopulation of CD4+ splenic T lymphocytes, DCs, and Tregs. These results indicated that PHPS plays key roles in immunoregulation and may be apply to develop new health foods. (C) 2017 Elsevier Ltd. All rights reserved.
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