Journal
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Volume 58, Issue 7, Pages 2832-2842Publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.16-20312
Keywords
retina; microaneurysms; cellular senescence; p16; p53; p21
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Funding
- Instituto de Salud Carlos III, Spain [PI12/00605, PI16/00719]
- Gundacao para a Ciencia e a Tecnologia [SFRH/BPD/102573/2014]
- Ministerio da Educacao e Ciencia, Portugal
- Fondo Europeo de Desarrollo Regional (FEDER)
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PURPOSE. Microaneurysms are present in healthy old-age human retinas. However, to date, no age-related pathogenic mechanism has been implicated in their formation. Here, cellular senescence, a hallmark of aging and several age-related diseases, has been analyzed in the old-age human retina and in the retina of a progeric mouse. METHODS. Retinas were obtained from 17 nondiabetic donors and from mice deficient in Bmi1. Cellular senescence was analyzed by immunohistochemistry, senescent-associated beta-galactosidase activity assay, Sudan black B staining, conventional transmission electron microscopy, and immunoelectronmicroscopy. RESULTS. Neurons, but not neuroglia, and blood vessels undergo cellular senescence in the old-age human retina. The canonical senescence markers p16, p53, and p21 were up-regulated and coexisted with apoptosis in old-age human microaneurysms. Senescent endothelial cells were discontinuously covered by fibronectin, and p16 colocalized with the beta(1) subunit of fibronectin receptor alpha(5)beta(1) integrin under the endothelial cellular membrane, suggesting anoikis as a mechanism involved in endothelial cell apoptosis. In a progeric mouse model deficient in Bmi1, where p21 was overexpressed, the retinal blood vessels displayed an aging phenotype characterized by enlarged caveolae and lipofuscin accumulation. Although mouse retina is not prone to develop microaneurysms, Bmi1-deficient mice presented abundant retinal microaneurysms. CONCLUSIONS. Together, these results uncover cellular senescence as a player during the formation of microaneurysms in old-age human retinas.
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