Adipose tissue is influenced by hypoxia of obstructive sleep apnea syndrome independent of obesity

Aims. - Obstructive sleep apnea syndrome (OSAS) is associated with increased cardiovascular risk and diabetes independent of obesity. We investigated whether adipose tissue dysfunction is exacerbated due to increased tissue hypoxia. Methods. - Adipose tissue (AT) oxygenation was measured with a Clarke-type electrode (PATO2) in 16 men with OSAS before and after 4 months of continuous positive airway pressure therapy (CPAP) and in BMI-matched controls. Oxygenation was simultaneously monitored in arterial blood by pulse oximetry (SaO(2)); mixed blood in AT microcirculation by reflectance spectroscopy (SATO2) along with blood flow. Markers of hypoxia, adipo- and angiogenesis, inflammation and fibrosis were analysed in AT and serum. Results. - OSAS subjects were more insulin resistant. Despite lower arterial SaO(2) (95.4 +/- 1.3% vs. 97.1 +/- 1.6%, P = 0.013) in subjects with OSAS, there was no difference in the oxygen content of AT microcirculation (61.6 +/- 18.4 vs. 72.2 +/- 7.0%, P = 0.07) or PATO2 (49.2 +/- 7.5 vs. 50.4 +/- 14.7 mmHg, P = 0.83) between groups. Resting AT blood flow was higher in OSAS compared to controls (108.5 +/- 22.7 vs. 78.9 +/- 24.9 au, P < 0.005) and strongly associated with inflammation markers IL-6 and MCP-1. AT of OSAS subjects showed increased inflammation (TNFA P = 0.049) and fibrosis (COL3A1 P = 0.02), a trend of higher HIF1A expression (P = 0.06) and reduced adipogenesis (PPARG P = 0.006). After CPAP, only expression of the lipid deposition marker LPL increased (30%, P= 0.047). Conclusions. - Adipose tissue of awake OSAS subjects appears no more hypoxic than adipose tissue of BMI-matched controls despite daytime hypoxaemia. Increased adipose tissue blood flow may be explained by an increased inflammatory response. We observe features of adipose dysfunction in subjects with OSAS, which attribute to increased cardiometabolic risk associated with this condition. (C) 2017 Elsevier Masson SAS. All rights reserved.