Journal
SCIENTIFIC REPORTS
Volume 7, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-14522-6
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Funding
- French Agence Nationale de la Recherche [ANR-14-CE14-0001]
- Zikalliance project (European Union - Horizon programme) [734548]
- European Virus Archive goes global project (EVAg
- European Union - Horizon programme) [653316]
- Zoonotic Anticipation and Preparedness Initiative (ZAPI project) [115760]
- IMI
- European Commission
- Agence Nationale de la Recherche (ANR) [ANR-14-CE14-0001] Funding Source: Agence Nationale de la Recherche (ANR)
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Reverse genetics is a critical tool to decrypt the biological properties of arboviruses. However, whilst reverse genetics methods have been usually applied to vertebrate cells, their use in insect cells remains uncommon due to the conjunction of laborious molecular biology techniques and of specific difficulties surrounding the transfection of such cells. To leverage reverse genetics studies in both vertebrate and mosquito cells, we designed an improved DNA transfection protocol for insect cells and then demonstrated that the simple and flexible ISA (Infectious Subgenomic Amplicons) reversegenetics method can be efficiently applied to both mammalian and mosquito cells to generate in days recombinant infectious positive-stranded RNA viruses belonging to genera Flavivirus (Japanese encephalitis, Yellow fever, West Nile and Zika viruses) and Alphavirus (Chikungunya virus). This method represents an effective option to potentially overcome technological issues related to the study of arboviruses.
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