Journal
DEVELOPMENT
Volume 144, Issue 11, Pages 2092-2097Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.145805
Keywords
Tissue clearing; 3D imaging; Chicken embryo; Light-sheet microscopy
Categories
Funding
- Human Frontier Science Program [RGP0004/2013]
- European Commission Seventh Framework Programme (FP7, EU CIG Grant)
- Ministerio de Economia y Competitividad [FIS2013-41802-R]
- Consejeria de Educacion, Juventud y Deporte, Comunidad de Madrid [P2013/ICE 2958]
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The CUBIC tissue-clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light-sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell resolution in whole early-stage chicken embryos and in the whole brains of late-stage embryos.
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