Journal
AGING-US
Volume 9, Issue 6, Pages 1585-1594Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/aging.101254
Keywords
CircRNA_100269; MiR-630; gastric cancer; proliferation
Categories
Funding
- National Natural Science Foundation of China [81672446, 81600510]
- Guangdong Provincial Science and Technology Key Project [2014A020215014]
- Research Fund of Public Welfare in the Health Industry
- National Health and Family Planning Commission of China [201402015]
- Key Clinical Specialty Discipline Construction Program
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Although CircRNA_100269 is a biomarker used to predict cancer recurrence, its expression and function in gastric cancer (GC) remain unknown. In this study, the expression of circRNA_100269 and its potential downstream miRNA targets were investigated. The molecular function and regulatory mechanism of circRNA_100269 in GC cell lines were also elucidated. The expression levels of circRNA_100269 and its linear isomer LPHN2 mRNA were found to be downregulated (p<0.01) in GC tissues. The target miRNA was predicted to be miR-630, whose expression was upregulated (p<0.01) and found to be negatively correlated with that of circRNA_100269 (r = -0.688) in GC tissues. Moreover, direct interaction of circRNA_100269 and miR-630 was confirmed through dual-luciferase assays. Overexpressing the circRNA_100269 plasmid inhibited cell proliferation (p<0.05). Furthermore, transfection of miR-630 mimics into cell lines overexpressing circRNA_100269 blocked the function of circRNA_100269 (p<0.05). Thus, circRNA_100269 level was downregulated in GC and correlated negatively with that of miR-630. Taken together, our results suggest that circRNA_100269 and miR-630 comprise a novel pathway that regulates proliferation of GC cells.
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