Journal
CROP PROTECTION
Volume 97, Issue -, Pages 135-144Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cropro.2016.11.012
Keywords
Biocontrol agent; Spore; Biocontrol; Hyperparasite; Biopesticide; Biofungicide
Categories
Funding
- European Community's Seventh Framework Programme [265865]
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A strain of Ampelomyces quisqualis (ITA3) was found to be more effective against powdery mildew than the commercial one (CNCM 1-807), however its mass production and formulation need to be developed for practical application as biopesticide. Strains of A. quisqualis are considered to be slow growing fungi with a radial growth rate of a few millimetres a week on solid media and their conidia are known to be difficult to produce in industrial fermenters. The current study optimised nutrient composition and growth conditions for high mycelial growth and conidiation of the ITA3 strain on a cost-effective culture medium and finalised a stable and effective dry formulation for the conidia. Plackett Burman design and response surface methodology were employed for optimisation of new bioprocesses. In the tested culture media, a 23.2-fold increase in biomass and a 2.2 x 10(8) conidia yield were obtained with the optimised sugar-based medium, with a time reduction of 43% as compared to original sugar-based medium. The kinetic parameter biomass yield (Y-x/(s))and specific growth rate (mu) values obtained were 3.29 g cells g(-1) substrate and 0.56 days(-1), respectively. When fermentation was scaled-up in a fermenter, 3.8 x 10(7) conidia mL(-1) were produced within 15 days. Silica gel powder added to undried conidia in water allowed good storage stability and preserved the biocontrol efficacy of the fungus. The high conidia yield and good biocontrol results achieved in this study with formulated ITA3 conidia are economically sustainable for the industrial production of A. quisqualis. (C) 2016 Elsevier Ltd. All rights reserved.
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