Journal
BIO-PROTOCOL
Volume 7, Issue 11, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2328
Keywords
Cyanobacteria; Synechocystis; Motility; Phototaxis; Photoreceptors
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Funding
- Excellence Initiative of the German Research Foundation (GSC-4, Spemann Graduate School)
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Phototaxis is a mechanism that allows cyanobacteria to respond to fluctuations in the quality and quantity of illumination by moving either towards or away from a light source. Phototactic movement on low concentration agar or agarose plates can be analyzed at macroscopic and microscopic scales representing group behavior and single cell motility, respectively. Here, we describe a detailed procedure for phototaxis assays on both scales using the unicellular cyanobacterium Synechocystis sp. PCC 6803.
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