Journal
BIO-PROTOCOL
Volume 7, Issue 11, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2308
Keywords
LDH assay; Primary neurons; Cell damage; LDH release; Cell culture; Cell death; Survival; Oxygen-glucose deprivation; Glutamate toxicity
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Funding
- German Research Foundation [SFB TRR43, HA5741/1-2]
- Federal Ministry of Education and Research [01 EO 08 01]
- Berlin Institute of Health [TRG7, TP1]
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The aim of many in vitro models of acute or chronic degenerative disorders in the neurobiology field is the assessment of survival or damage of neuronal cells. Damage of cells is associated with loss of outer cell membrane integrity and leakage of cytoplasmic cellular proteins. Therefore, activity assays of cytoplasmic enzymes in supernatants of cell cultures serve as a practicable tool for quantification of cellular injury (Koh and Choi, 1987; Bruer et al., 1997). Lactate dehydrogenase (LDH) is such a ubiquitously expressed cytosolic enzyme, which is very stable due to a very long protein half-life (Hsieh and Blumenthal, 1956; Koh and Cotman, 1992; Koh et al., 1995).
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