Journal
CURRENT SCIENCE
Volume 112, Issue 10, Pages 2073-2084Publisher
INDIAN ACAD SCIENCES
DOI: 10.18520/cs/v112/i10/2073-2084
Keywords
Genetic differentiation; leaf rust; microsatellites; Puccinia triticina; virulence phenotype
Categories
Funding
- Department of Biotechnology, Government of India [BT/PR13631/AGR/02/717-C/2010]
Ask authors/readers for more resources
Leaf or brown rust caused by Puccinia triticina (Pt) is one of the most important diseases of wheat. Among the rusts, it is the most ubiquitous in all the wheatgrowing regions and causes considerable yield loss. Microsatellite marker-based genotyping and virulence-based phenotyping of 48 pathotypes of Pt was performed. The pathotypes exhibit low virulence frequencies for Indian leaf rust differentials Lr24, Lr9, Lr10, Lr19, Lr28 and Lr9. Using avirulence/virulence formula six major clusters of pathotypes were observed, revealing high degree of phenotypic variation. Molecular analysis performed using SSR markers showed high genetic diversity among the pathotypes, and grouped them in seven major clusters. The percentage of polymorphic loci ranged from 17.95 to 84.62, Nei's gene diversity from 0.07 to 0.32 and Shannon's information index from 0.11 to 0.47. Analysis of molecular variance revealed significantly high genetic variation within Pt population. Mantel's Z test proved low positive correlation (r = 0.28) between virulence and molecular diversity, suggesting independent nature of the duo. These findings offer valuable information for framing suitable disease management strategies through appropriate region-specific gene deployment and improve the understanding of the population biology and evolution of Pt in the Indian subcontinent.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available