A new fluorescent PET sensor probe for Co2+ ion detection: computational, logic device and living cell imaging applications

The hydrophobic nature of beta-cyclodextrin (beta-CD) increases the solubility and stability of 1,2-dihydroxyanthraquinone (1,2-DHAQ; 1) and hence permits the coordination of 1 with cations in aqueous solution; this has been investigated by means of UV-visible and fluorescence spectroscopy. beta-CD: 1,2-DHAQ ( 2) demonstrates colorimetric recognition behavior toward Co2+ ion by changing the color of the solution, which can be easily detected with the naked eye. The chemosensor 2 showed good fluorescence behavior upon interaction with various cations; it displayed strong fluorescence quenching (Co2+; similar to 80% switch-off) as a fluorescent chemosensor based on photoinduced electron transfer (PET). Moreover, when the 2 center dot Co2+ complex was tested with various anions, only nitrate (NO3- ) enabled cobalt binding (as CoNO3) and led to fluorescence enhancement (similar to 82%; switch-on). The detection limits of sensor 2 with Co2+ and NO3- were found to be 22.7 nM and 2.4 nM. Theoretical molecular docking studies and density functional theory (DFT) calculations were performed to study the binding of Co2+ and NO3- ions with 2. The changes in the fluorescence of 2 upon addition of Co2+ followed by NO3- can be utilised as an XNOR logic gate. Furthermore, 2 has potential for use in bio-imaging as a fluorescent probe to detect Co2+ ion, followed by sequential detection of NO3- ion by 2 center dot Co2+, in living cells (human cervical cancer HeLa cell line) using confocal laser scanning microscopy.