Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 56, Issue 25, Pages 7146-7150Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201702669
Keywords
amino acids; dynamics; isotopic labeling; mass spectrometry imaging; metabolism
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Funding
- Dutch Province of Limburg as part of the LINK program
- Worldwide Cancer Research (WCR) [15-0345]
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Mass spectrometry imaging (MSI) simultaneously detects and identifies the spatial distribution of numerous molecules throughout tissues. Currently, MSI is limited to providing a static and ex vivo snapshot of highly dynamic systems in which molecules are constantly synthesized and consumed. Herein, we demonstrate an innovative MSI methodology to study dynamic molecular changes of amino acids within biological tissues by measuring the dilution and conversion of stable isotopes in a mouse model. We evaluate the method specifically on hepatocellular metabolism of the essential amino acid l-phenylalanine, associated with liver diseases. Crucially, the method reveals the localized dynamics of L-phenylalanine metabolism, including its in vivo hydroxylation to L-tyrosine and co-localization with other liver metabolites in a time course of samples from different animals. This method thus enables the dynamics of localized biochemical synthesis to be studied directly from biological tissues.
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