Journal
RSC ADVANCES
Volume 7, Issue 40, Pages 24789-24794Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra01815k
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Funding
- National Natural Science Foundation of China (NNSFC) [81460327, 21105010]
- Science and Technology Agency of Jiangxi Province [20152ACG70014]
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Rapid recognition of Staphylococcal bacteremia in humans is a serious challenge in clinical research. Here we report on direct analysis of molecular volatile organic compounds (VOCs) of blood cultures emitted by 35 patients with a suspicion of Staphylococcal bacteremia and 31 patients positively diagnosed with Staphylococcal bacteremia were fingerprinted by atmospheric corona discharge ionization mass spectrometry (CDI-MS) for the presence of four common Staphylococci (Staphylococcus aureus, S. hominis, S. haemolyticus, and S. epidermidis) which statistically account for ca. 90% of cases of Staphylococcal bacteremia in humans. All the infected blood cultures revealed characteristic and clearly distinct MS patterns specific to the presence of one of the four Staphylococci after an incubation time of 3-16 h. Technical replicates were incubated over 2-3 days for reference diagnosis using current conventional bacteremia detection. The results of our 6 month hospital study confirm peaks at m/z 87 (isobutyric acid) and m/z 101 (isovaleric acid) signals were specifically present in Staphylococci, and indicate that high suitability of CDI-MS for rapid, reliable and cost-efficient diagnosis of Staphylococcal bacteremia in humans.
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