Journal
RSC ADVANCES
Volume 7, Issue 81, Pages 51324-51334Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra10756k
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Funding
- German Research Foundation (DFG) [STE640/14]
- Federal Ministry of Education and Research of Germany (BMBF) [05K14MPA]
- German Academic Exchange Service (DAAD)
- Russian Science Foundation [14-50-00029]
- Russian Foundation for Basic Research (RFBR) [15-54-12385, 16-02-00248]
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The styrene-maleic acid (SMA) copolymers enable a detergent-free extraction of membrane proteins from lipid bilayers yielding stable water-soluble nanocontainers with lipid-encased proteins. Here, we demonstrate the ability of the SMA copolymer to extract a complex of interacting membrane proteins from proteoliposomes, namely the archaeal sensory rhodopsin II from Natronomonas pharaonis, NpSRII, in complex with its transducer, NpHtrII. The NpSRII/NpHtrII complexes retained their structural integrity within SMA lipid particles as revealed by diverse biophysical techniques. Pulse electron paramagnetic resonance data showed the SMA-encased NpSRII/NpHtrII complexes as native-like 2 : 2 dimers with a distance between NpSRII sites 159 according to the V-shaped conformation found in a crystal structure.
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