Journal
RSC ADVANCES
Volume 7, Issue 87, Pages 55199-55205Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra11835j
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21765015, 21461015]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC201602]
- Science Foundation of Jiangxi Province [KJLD14010, 20153BCB23035]
- Jiangxi Province Food and Drug Administration Science Foundation, China [2016SP04]
Ask authors/readers for more resources
In this study, a highly sensitive fluorescent probe based on bovine serum protein-protected gold nanoclusters (BSA-AuNCs) was developed for the determination of organophosphorus pesticides (OPs). At first, BSA-AuNCs were prepared using a photo stable fluorescent substrate. Acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine iodide (ATCI) to produce thiocholine (TCh), and the sulfhydryl groups (SH-) on TCh form Au-S bonds with BSA-AuNCs; thus, the fluorescence of BSA-AuNCs weakens. On the other hand, OPs can inhibit the activity of AChE, thus preventing the generation of TCh, and the fluorescence recovery of BSA-AuNCs occurs. Under optimized experimental conditions, parathion-methyl (PM) was detected in the concentration range of 0.33-6.67 ng mL(-1) with a detection limit of 0.14 ng mL(-1) (S/N = 3), which is much lower than the maximum residue limits reported in the European Union pesticides database as well as that defined by the U.S. Department Agriculture. Moreover, the assay demonstrated highly sensitive applications in the quantitative determination of OPs in water and food samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available