4.6 Article

System-wide analysis of manganese starvation-induced metabolism in key elements of Lactobacillus plantarum

Journal

RSC ADVANCES
Volume 7, Issue 21, Pages 12959-12968

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra00072c

Keywords

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Funding

  1. State Key Program of National Natural Science Foundation of China [31530056]
  2. Science and Nature Foundation of Jiangsu Province [BK 20160169]
  3. Jiangsu Province Postdoctoral Science Foundation [1601183C]
  4. China Postdoctoral Science Foundation [2015M581727]
  5. Program of Introducing Talents of Discipline to Universities [B07029]
  6. Program for Changjiang Scholars and Innovative Research Team in University [IRT1249]
  7. Program of Collaborative innovation center of food safety and quality control in Jiangsu Province

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To analyze the response mechanisms of Lactobacillus plantarum against manganese starvation stress, different metabolisms from physiology, proteomics and transporters aspects in L. plantarum CCFM 436 were systematically investigated. The kinetics of cell growth (mu(max)) decreased from 0.310 to 0.256 h(-1), while thinner cell morphology was observed by transmission electron microscopy under Mn-starvation conditions. Gas chromatography-mass spectrometry analysis indicated that membrane mobility and compactness increased, with a higher proportion of unsaturated fatty acids and cyclopropane fatty acids. High-performance liquid chromatography analysis showed that intracellular Asp, Glu, and Arg contents, closely related to energy metabolism, were significantly increased. Fourier transform infrared spectroscopy proved that some functional groups (N-H and O=C-OH) were significantly affected by Mn starvation. Comparative two-dimensional proteomic analysis identified 73 proteins that differed significantly under Mn starvation conditions. These differentially expressed proteins involved in carbohydrate, amino acid and transcription/translation metabolisms and stress response were categorized as crucial components required to resist manganese starvation stress. Moreover, qRT-PCR analysis proved that MntH 1-5, negatively regulated by MntR, acted as potential Mn importers under Mn starvation conditions. The proposed coordinated mechanism model provides a reference for, and insight into, the intracellular metabolism of LAB strains.

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