Journal
GENOME BIOLOGY
Volume 18, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/s13059-017-1237-8
Keywords
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Funding
- Skoltech Center
- NCI in the MIT-Harvard Center of Cancer Nanotechnology Excellence [5-U54-CA151884-04]
- China Scholarship Council [201506260151]
- Chinese government
- NIH [DP2HL137167, P01HL131471, R00CA169512, GM115911]
- American Cancer Society [129056-RSG-16-093]
- Lung Cancer Research Foundation
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CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of beta-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of beta-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.
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